Hotspot

rpl22-rps3

Genome · 2895-3001 · 107 bp

Back to Dryopteris

Pi 0.0498

MarkerSeek score 72.80

Primer available yes

Label rpl22-rps3

Feature Metadata

label_name: rpl22-rps3
feature_type: igs
region: Genome
length_bp: 107
variable_sites: 10
pi: 0.0498
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps3_p1	TGTAACTGATCGCATGGTGG	TGTCTGTGCGAACCGATAAG	50.0	50.0	58.0	58.0	676-677 bp	1.0	83.3
rpl22-rps3_p2	TGTAACTGATCGCATGGTGG	TTGAGTCGTCCGGCTATTTG	50.0	50.0	58.0	58.0	1104-1105 bp	1.0	82.6
rpl22-rps3_p3	TGTAACTGATCGCATGGTGG	GTTGAGTCGTCCGGCTATTT	50.0	50.0	58.0	58.0	1105-1106 bp	1.0	82.6
rpl22-rps3_p4	TGTAACTGATCGCATGGTGG	CTTGTCTGTGCGAACCGATA	50.0	50.0	58.0	58.0	679 bp	0.75	71.5
rpl22-rps3_p5	TTCATAATGGCCGAGAGCAC	TTGAGTCGTCCGGCTATTTG	50.0	50.0	58.0	58.0	1136 bp	0.25	49.6