Hotspot

trnI-CAU-rpl23

Genome · 121-285 · 165 bp

Back to Dryopteris

Pi 0.0626
MarkerSeek score 75.90
Primer available yes
Label trnI-CAU-rpl23

Feature Metadata

label_name
trnI-CAU-rpl23
feature_type
igs
region
Genome
length_bp
165
variable_sites
19
pi
0.0626
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-rpl23_p1 TAATTGGCGAATTCACGGGT TGGTTCTTCAATTTCTCCGT 45.0 40.0 57.9 54.9 224 bp 1.0 61.0
trnI-CAU-rpl23_p2 ATTCACGGGTTCAACTCCTG TGGTTCTTCAATTTCTCCGT 50.0 40.0 57.8 54.9 214 bp 1.0 60.7
trnI-CAU-rpl23_p3 TAATTGGCGAATTCACGGGT TTGGTTCTTCAATTTCTCCGT 45.0 38.1 57.9 55.7 225 bp 1.0 59.5
trnI-CAU-rpl23_p4 ATTCACGGGTTCAACTCCTG TTGGTTCTTCAATTTCTCCGT 50.0 38.1 57.8 55.7 215 bp 1.0 59.3
trnI-CAU-rpl23_p5 CGGTTAAAGCACCCAACTCA TGGTTCTTCAATTTCTCCGT 50.0 40.0 58.4 54.9 244 bp 1.0 58.1