Hotspot

rps16

LSC · 5027-6159 · 1133 bp

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Pi 0.00235

MarkerSeek score 60.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1133
variable_sites: 4
pi: 0.00235
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAGTAAGACGGTGGAGAAGT	TGTACCAACCGAACCAATGA	45.0	45.0	55.8	57.1	1299-1301 bp	1.0	55.4
rps16_p2	AGTAAGACGGTGGAGAAGTAGA	TGTACCAACCGAACCAATGA	45.5	45.0	57.7	57.1	1298-1300 bp	1.0	54.5
rps16_p3	AAGTAAGACGGTGGAGAAGT	TGGGTTCCAAACTGAAGGAA	45.0	45.0	55.8	56.9	1245-1247 bp	1.0	53.8
rps16_p4	AAGACGGTGGAGAAGTAGAA	TGTACCAACCGAACCAATGA	45.0	45.0	55.5	57.1	1295-1297 bp	1.0	53.0
rps16_p5	AGACGGTGGAGAAGTAGAAA	TGTACCAACCGAACCAATGA	45.0	45.0	55.5	57.1	1294-1296 bp	1.0	53.0