Hotspot

ndhD

SSC · 115682-117184 · 1503 bp

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Pi 0.000887

MarkerSeek score 59.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 2
pi: 0.000887
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGAACGATTTTTAAAAGCATCGGA	CTTTACCGACAAAAACCCGC	33.3	50.0	58.0	58.0	1643 bp	1.0	47.7
ndhD_p2	TGAACGATTTTTAAAAGCATCGG	CTTTACCGACAAAAACCCGC	34.8	50.0	56.7	58.0	1643 bp	1.0	45.2
ndhD_p3	TGAACGATTTTTAAAAGCATCGGA	AGCACGGGTTTTTATGGTCC	33.3	50.0	58.0	58.5	1602 bp	1.0	44.4
ndhD_p4	TGAACGATTTTTAAAAGCATCGGA	CAAAAACCCGCGCTCAAAAT	33.3	45.0	58.0	58.5	1634 bp	1.0	44.0
ndhD_p5	ACGATTTTTAAAAGCATCGGA	CTTTACCGACAAAAACCCGC	33.3	50.0	54.5	58.0	1640 bp	1.0	43.7