Hotspot

ndhF-rpl32

SSC · 112850-113448 · 599 bp

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Pi 0.00342

MarkerSeek score 65.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 599
variable_sites: 3
pi: 0.00342
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAACGGT	AAAAGCTTTCAATGCTGCCC	50.0	45.0	57.8	57.8	705-747 bp	1.0	81.5
ndhF-rpl32_p2	CTGGAAGTGGAATGAACGGT	ATGCTGCCCAATACCCTTTC	50.0	50.0	57.8	58.2	694-736 bp	1.0	81.1
ndhF-rpl32_p3	CTGGAAGTGGAATGAACGGT	AATGCTGCCCAATACCCTTT	50.0	45.0	57.8	57.7	695-737 bp	1.0	80.8
ndhF-rpl32_p4	CTGGAAGTGGAATGAACGGT	CAATGCTGCCCAATACCCTT	50.0	50.0	57.8	58.5	696-738 bp	1.0	78.8
ndhF-rpl32_p5	CTGGAAGTGGAATGAACGGT	TTCAATGCTGCCCAATACCC	50.0	50.0	57.8	58.5	698-740 bp	1.0	78.7