Hotspot

psaI-ycf4

LSC · 59573-59996 · 424 bp

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Pi 0.00472

MarkerSeek score 48.00

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 3
pi: 0.00472
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TACGTTCTGATCGCCAACTC	45.0	50.0	58.0	57.7	507-509 bp	1.0	78.1
psaI-ycf4_p2	GGGCTTAGTATTTCCGGCAA	TACGTTCTGATCGCCAACTC	50.0	50.0	58.0	57.7	516-518 bp	1.0	77.9
psaI-ycf4_p3	CGGCAATTGCAATGGTTTCT	TACGTTCTGATCGCCAACTC	45.0	50.0	57.9	57.7	502-504 bp	1.0	77.6
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	CGTTCTGATCGCCAACTCAT	45.0	50.0	58.0	58.4	505-507 bp	1.0	77.4
psaI-ycf4_p5	GGGCTTAGTATTTCCGGCAA	CGTTCTGATCGCCAACTCAT	50.0	50.0	58.0	58.4	514-516 bp	1.0	77.3