Hotspot

rbcL-accD

LSC · 57038-57687 · 650 bp

Back to Drymocallis

Pi 0.00103

MarkerSeek score 59.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGCTCGTGAGGGTAATGAAA	CGTTAGCTTACGCCCGTATT	50.0	50.0	58.0	58.2	833-834 bp	1.0	78.9
rbcL-accD_p2	CGCTCGTGAGGGTAATGAAA	AACAAGAGCGAACCAAACCA	50.0	45.0	58.0	58.2	799-800 bp	1.0	78.2
rbcL-accD_p3	CGCTCGTGAGGGTAATGAAA	CGAACCAAACCACCGTTTTT	50.0	45.0	58.0	57.7	791-792 bp	1.0	77.9
rbcL-accD_p4	CGCTCGTGAGGGTAATGAAA	ACCAAACCACCGTTTTTCCA	50.0	45.0	58.0	58.4	788-789 bp	1.0	76.6
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	CGTTAGCTTACGCCCGTATT	55.0	50.0	57.7	58.2	791-792 bp	1.0	76.6