Hotspot

ycf3-trnS-GGA

LSC · 44775-45469 · 695 bp

Back to Drymocallis

Pi 0.00192

MarkerSeek score 62.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 2
pi: 0.00192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	638-879 bp	1.0	70.3
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	639-880 bp	1.0	70.3
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	588-829 bp	1.0	70.0
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	589-830 bp	1.0	70.0
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	AGTTCCAATGCTACGCCTTC	45.0	50.0	56.4	58.3	590-831 bp	1.0	69.5