Hotspot

psaA-ycf3

LSC · 41983-42738 · 756 bp

Back to Drymocallis

Pi 0.00176

MarkerSeek score 60.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 2
pi: 0.00176
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTACGGGATCCCTATCGACC	GCTATAGCGCTTACTCCTGG	55.0	55.0	58.1	57.6	885-886 bp	1.0	76.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	907-908 bp	1.0	76.6
psaA-ycf3_p3	TTACGGGATCCCTATCGACC	GGAGGCTTGGTTCGATCAAG	55.0	55.0	58.1	58.6	925-926 bp	1.0	74.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	50.0	55.0	57.9	58.6	947-948 bp	1.0	74.4
psaA-ycf3_p5	TTACGGGATCCCTATCGACC	TGGAAACAAGCTATAGCGCT	55.0	45.0	58.1	57.3	894-895 bp	1.0	74.1