Hotspot

trnE-UUC-trnT-GGU

LSC · 30912-31440 · 529 bp

Back to Drymocallis

Pi 0.00252
MarkerSeek score 58.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
529
variable_sites
2
pi
0.00252
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGAGATGTCCTGGACCAC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 618 bp 1.0 79.1
trnE-UUC-trnT-GGU_p2 GAGAGATGTCCTGGACCACT GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 617 bp 1.0 79.1
trnE-UUC-trnT-GGU_p3 AGAGAGATGTCCTGGACCAC CCATGGCGTTACTCTACCAC 55.0 55.0 58.1 58.1 597 bp 1.0 78.6
trnE-UUC-trnT-GGU_p4 GAGAGATGTCCTGGACCACT CCATGGCGTTACTCTACCAC 55.0 55.0 58.1 58.1 596 bp 1.0 78.6
trnE-UUC-trnT-GGU_p5 GAGAGATGTCCTGGACCACT TGGCGTTACTCTACCACTGA 55.0 50.0 58.1 58.1 593 bp 1.0 78.5