Hotspot

petN-psbM

LSC · 28272-29544 · 1273 bp

Back to Drymocallis

Pi 0.0021

MarkerSeek score 60.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1273
variable_sites: 4
pi: 0.0021
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	GTAAAAACAGTCAGCCAAGGC	50.0	47.6	58.0	58.0	1379-1382 bp	1.0	72.2
petN-psbM_p2	AGTAAATCTCGCTTGGGCTG	CGTAAAAACAGTCAGCCAAGG	50.0	47.6	58.0	57.7	1380-1383 bp	1.0	70.1
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	GTAAAAACAGTCAGCCAAGGC	50.0	47.6	57.6	58.0	1319-1322 bp	1.0	69.6
petN-psbM_p4	AGTAAATCTCGCTTGGGCTG	TAAAAACAGTCAGCCAAGGC	50.0	45.0	58.0	56.5	1378-1381 bp	1.0	68.6
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	CGTAAAAACAGTCAGCCAAGG	50.0	47.6	57.6	57.7	1320-1323 bp	1.0	67.6