Hotspot

trnR-UCU-atpA

LSC · 9899-10543 · 645 bp

Back to Drymocallis

Pi 0.00517

MarkerSeek score 51.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 645
variable_sites: 5
pi: 0.00517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATTGGAGTGAAAAGCGTCCA	AAGACGTTTACCGAGGAAGC	45.0	50.0	57.7	57.9	811-818 bp	1.0	79.0
trnR-UCU-atpA_p2	TGGAGTGAAAAGCGTCCATT	AAGACGTTTACCGAGGAAGC	45.0	50.0	57.7	57.9	809-816 bp	1.0	79.0
trnR-UCU-atpA_p3	TTGGAGTGAAAAGCGTCCAT	AAGACGTTTACCGAGGAAGC	45.0	50.0	57.7	57.9	810-817 bp	1.0	79.0
trnR-UCU-atpA_p4	TGGAGTGAAAAGCGTCCATT	GAGGAAGCGGAAACCCTTTT	45.0	50.0	57.7	58.4	797-804 bp	1.0	76.6
trnR-UCU-atpA_p5	TTGGAGTGAAAAGCGTCCAT	GAGGAAGCGGAAACCCTTTT	45.0	50.0	57.7	58.4	798-805 bp	1.0	76.6