Hotspot

trnA-UGC-trnI-GAU

IRa · 132905-132974 · 70 bp

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Pi 0.143

MarkerSeek score 86.80

Primer available yes

Label trnA-UGC-trnI-GAU

Feature Metadata

label_name: trnA-UGC-trnI-GAU
feature_type: igs
region: IRa
length_bp: 70
variable_sites: 10
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-trnI-GAU_p1	CAACGACCCAATTGCAAGAG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	167-168 bp	1.0	82.0
trnA-UGC-trnI-GAU_p2	CAACGACCCAATTGCAAGAG	AGGTCTCTGGTTCAAGTCCA	50.0	50.0	57.7	57.9	149-150 bp	1.0	81.8
trnA-UGC-trnI-GAU_p3	CCGCCTGGACAATTAGACAT	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	201-202 bp	1.0	81.3
trnA-UGC-trnI-GAU_p4	CCGCCTGGACAATTAGACAT	AGGTCTCTGGTTCAAGTCCA	50.0	50.0	57.7	57.9	183-184 bp	1.0	80.9
trnA-UGC-trnI-GAU_p5	AGACATCCAACCCGTAATCG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.4	58.0	187-188 bp	1.0	79.5