Hotspot

rpl32-trnL-UAG

SSC · 111122-112092 · 971 bp

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Pi 0.137

MarkerSeek score 75.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 971
variable_sites: 90
pi: 0.137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTATTGGGCGGCGTTAAAAG	CTCGCACTGCTTCCTAAGAG	50.0	55.0	57.8	58.1	839-1124 bp	1.0	89.8
rpl32-trnL-UAG_p2	GTATTGGGCGGCGTTAAAAG	CTCTCGCACTGCTTCCTAAG	50.0	55.0	57.8	58.1	841-1126 bp	1.0	89.8
rpl32-trnL-UAG_p3	CGGAAAAAGGATAAGCCCCA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	57.9	58.1	1771 bp	0.5	58.3
rpl32-trnL-UAG_p4	CGGAAAAAGGATAAGCCCCA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	57.9	58.1	1773 bp	0.5	58.3
rpl32-trnL-UAG_p5	CGGAAAAAGGATAAGCCCCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1760 bp	0.5	57.9