Hotspot

trnI-GAU-trnA-UGC

IRb · 100677-100746 · 70 bp

Back to Drymaria

Pi 0.143

MarkerSeek score 86.80

Primer available yes

Label trnI-GAU-trnA-UGC

Feature Metadata

label_name: trnI-GAU-trnA-UGC
feature_type: igs
region: IRb
length_bp: 70
variable_sites: 10
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-trnA-UGC_p1	TGATTTACTTCACGGGCGAG	CAACGACCCAATTGCAAGAG	50.0	50.0	58.0	57.7	167-168 bp	1.0	82.0
trnI-GAU-trnA-UGC_p2	AGGTCTCTGGTTCAAGTCCA	CAACGACCCAATTGCAAGAG	50.0	50.0	57.9	57.7	149-150 bp	1.0	81.8
trnI-GAU-trnA-UGC_p3	TGATTTACTTCACGGGCGAG	CCGCCTGGACAATTAGACAT	50.0	50.0	58.0	57.7	201-202 bp	1.0	81.3
trnI-GAU-trnA-UGC_p4	AGGTCTCTGGTTCAAGTCCA	CCGCCTGGACAATTAGACAT	50.0	50.0	57.9	57.7	183-184 bp	1.0	80.9
trnI-GAU-trnA-UGC_p5	TGATTTACTTCACGGGCGAG	AGACATCCAACCCGTAATCG	50.0	50.0	58.0	57.4	187-188 bp	1.0	79.5