Hotspot

rpl36-rps8

LSC · 77961-78405 · 445 bp

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Pi 0.125

MarkerSeek score 81.80

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 51
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TTTTACGAACAGAAGCCCTT	TGGCGGAGAAATTTTATGTT	40.0	35.0	55.2	53.4	467-504 bp	1.0	52.0
rpl36-rps8_p2	TTTTACGAACAGAAGCCCTT	TTGGCGGAGAAATTTTATGT	40.0	35.0	55.2	53.4	468-505 bp	1.0	52.0
rpl36-rps8_p3	TTTTACGAACAGAAGCCCTT	TTGGCGGAGAAATTTTATGTT	40.0	33.3	55.2	54.3	468-505 bp	1.0	52.0
rpl36-rps8_p4	CGAACAGAAGCCCTTATTTTCA	TGGCGGAGAAATTTTATGTT	40.9	35.0	57.0	53.4	462-499 bp	1.0	52.0
rpl36-rps8_p5	CGAACAGAAGCCCTTATTTTCA	TTGGCGGAGAAATTTTATGT	40.9	35.0	57.0	53.4	463-500 bp	1.0	52.0