Hotspot

psbE-petL

LSC · 63030-64001 · 972 bp

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Pi 0.0922

MarkerSeek score 85.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 972
variable_sites: 86
pi: 0.0922
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CGGAAGTTATAGTTAAAGCTGCG	50.0	43.5	57.8	57.9	1057-1289 bp	1.0	62.7
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	CGGAAGTTATAGTTAAAGCTGCG	55.0	43.5	58.2	57.9	1052-1284 bp	1.0	62.4
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	CGGAAGTTATAGTTAAAGCTGCG	50.0	43.5	58.4	57.9	1050-1282 bp	1.0	61.2
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	CGGAAGTTATAGTTAAAGCTGCG	50.0	43.5	58.6	57.9	1051-1283 bp	1.0	59.4
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	CGGAAGTTATAGTTAAAGCTGCG	45.0	43.5	57.4	57.9	1058-1290 bp	1.0	59.0