Hotspot

petA-psbJ

LSC · 61256-62228 · 973 bp

Back to Drymaria

Pi 0.104

MarkerSeek score 78.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 82
pi: 0.104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GATGTTTCGGATGGACGTGA	TGATGAAATGCGGAGGGAAG	50.0	50.0	58.0	58.0	1294-1397 bp	1.0	89.2
petA-psbJ_p2	GATGTTTCGGATGGACGTGA	TGGGGTCAATATCCGCAATG	50.0	50.0	58.0	58.0	1619-1705 bp	1.0	89.0
petA-psbJ_p3	ATGTTTCGGATGGACGTGAG	TGGGGTCAATATCCGCAATG	50.0	50.0	58.0	58.0	1618-1704 bp	1.0	88.9
petA-psbJ_p4	GATGTTTCGGATGGACGTGA	GCGGAAGGACCTCGTTTAAT	50.0	50.0	58.0	58.0	1455 bp	0.5	59.8
petA-psbJ_p5	ATGTTTCGGATGGACGTGAG	GCGGAAGGACCTCGTTTAAT	50.0	50.0	58.0	58.0	1454 bp	0.5	59.8