Hotspot

trnF-GAA-ndhJ

LSC · 46706-47207 · 502 bp

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Pi 0.0832

MarkerSeek score 80.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 41
pi: 0.0832
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ACGTAAGGATTATATTGTACCCA	50.0	34.8	58.1	54.7	588-607 bp	1.0	46.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ACGTAAGGATTATATTGTACCCAA	50.0	33.3	58.1	55.4	588-607 bp	1.0	46.7
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ACGTAAGGATTATATTGTACCCA	55.0	34.8	57.9	54.7	612-631 bp	1.0	46.4
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ACGTAAGGATTATATTGTACCCA	55.0	34.8	58.0	54.7	624-643 bp	1.0	46.3
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ACGTAAGGATTATATTGTACCCAA	55.0	33.3	58.0	55.4	624-643 bp	1.0	46.3