Hotspot

psaA-ycf3

LSC · 39844-40918 · 1075 bp

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Pi 0.0943

MarkerSeek score 82.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 1075
variable_sites: 90
pi: 0.0943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTGGCCCATTCCTCAAATGA	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	1099-1190 bp	1.0	88.1
psaA-ycf3_p2	TCTGGCCCATTCCTCAAATG	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	1100-1191 bp	1.0	88.1
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	1101-1192 bp	1.0	88.1
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	1049-1140 bp	1.0	85.7
psaA-ycf3_p5	TGGCCCATTCCTCAAATGAA	ATTGGTTGAAGATCACGGGG	45.0	50.0	57.4	57.9	1098-1189 bp	1.0	84.3