Hotspot

trnS-GCU-trnG-UCC

LSC · 7928-8475 · 548 bp

Back to Drymaria

Pi 0.101

MarkerSeek score 82.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 51
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	619-657 bp	1.0	57.1
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	TTACCACTAAACTATACCCGC	50.0	42.9	58.2	54.9	620-658 bp	1.0	56.0
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TTTACCACTAAACTATACCCGC	50.0	40.9	58.2	55.6	621-659 bp	1.0	54.3
trnS-GCU-trnG-UCC_p4	CTTTCGTCCACTCAGCCATC	TACCACTAAACTATACCCGC	55.0	45.0	58.6	54.0	555-593 bp	1.0	53.9
trnS-GCU-trnG-UCC_p5	AACGGAAAGAGAGGGATTCG	TACCACTAAACTATACCCGC	50.0	45.0	57.3	54.0	620-658 bp	1.0	52.9