Hotspot

rps16-trnQ-UUG

LSC · 5909-6543 · 635 bp

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Pi 0.106
MarkerSeek score 83.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
635
variable_sites
64
pi
0.106
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GCGAAGAAACAGCTACTCGA 50.0 50.0 58.0 58.0 1161-1164 bp 1.0 89.0
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GCGAAGAAACAGCTACTCGA 50.0 50.0 58.0 58.0 1159-1162 bp 1.0 89.0
rps16-trnQ-UUG_p3 CAGCGGATCATGTCCTTCAA AACCAAGTAATGCGGGTTGT 50.0 45.0 58.0 58.0 1439 bp 0.5 59.7
rps16-trnQ-UUG_p4 GCGGATCATGTCCTTCAAGT AACCAAGTAATGCGGGTTGT 50.0 45.0 58.0 58.0 1437 bp 0.5 59.7
rps16-trnQ-UUG_p5 CCAACAAGAAATGCGGGTTC AACCAAGTAATGCGGGTTGT 50.0 45.0 57.9 58.0 1533 bp 0.5 59.4