Hotspot

trnH-GUG-psbA

LSC · 72-324 · 253 bp

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Pi 0.139

MarkerSeek score 80.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 253
variable_sites: 33
pi: 0.139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTAGCTGCTATCGAAGTTCC	55.0	52.4	58.1	58.0	311-330 bp	1.0	82.8
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTAGCTGCTATCGAAGTTCC	50.0	52.4	57.9	58.0	325-344 bp	1.0	82.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTAGCTGCTATCGAAGTTCC	50.0	52.4	57.9	58.0	324-343 bp	1.0	82.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTAGCTGCTATCGAAGTTCC	50.0	52.4	57.9	58.0	326-345 bp	1.0	82.4
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	AGACCTAGCTGCTATCGAAGT	55.0	47.6	58.1	58.1	314-333 bp	1.0	82.4