Hotspot

rps16

LSC · 5188-6343 · 1156 bp

Back to Dryas

Pi 0.00156
MarkerSeek score 60.10
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1156
variable_sites
4
pi
0.00156
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACAAGTCATTCACACCCTCT ACTGGTTCCAAACTGAAGGA 45.0 45.0 56.4 56.6 1213-1215 bp 1.0 56.0
rps16_p2 ACAAGTCATTCACACCCTCT TGTACTGACCGAACCAATGA 45.0 45.0 56.4 56.5 1266-1268 bp 1.0 55.3
rps16_p3 ACAAGTCATTCACACCCTCTT ACTGGTTCCAAACTGAAGGA 42.9 45.0 57.1 56.6 1213-1215 bp 1.0 54.1
rps16_p4 ACAAGTCATTCACACCCTCT ACTGGTTCCAAACTGAAGGAA 45.0 42.9 56.4 57.3 1213-1215 bp 1.0 54.1
rps16_p5 ACAAGTCATTCACACCCTCTT TGTACTGACCGAACCAATGA 42.9 45.0 57.1 56.5 1266-1268 bp 1.0 53.4