Hotspot

psbA

LSC · 268-1329 · 1062 bp

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Pi 0.000377
MarkerSeek score 36.20
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
1
pi
0.000377
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 AGGAGCAATACCAAACCTCT AGAATTCGTGTGCTTGGGAG 45.0 50.0 56.1 58.2 1208 bp 1.0 63.5
psbA_p2 AGGAGCAATACCAAACCTCT AGAGAATTCGTGTGCTTGGG 45.0 50.0 56.1 58.2 1210 bp 1.0 63.5
psbA_p3 AGGAGCAATACCAAACCTCT GAGAATTCGTGTGCTTGGGA 45.0 50.0 56.1 58.2 1209 bp 1.0 63.5
psbA_p4 AGGAGCAATACCAAACCTCTT AGAATTCGTGTGCTTGGGAG 42.9 50.0 56.9 58.2 1208 bp 1.0 61.8
psbA_p5 AGGAGCAATACCAAACCTCTT AGAGAATTCGTGTGCTTGGG 42.9 50.0 56.9 58.2 1210 bp 1.0 61.8