Hotspot

ndhF

SSC · 113450-115705 · 2256 bp

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Pi 0.00124
MarkerSeek score 43.50
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2256
variable_sites
7
pi
0.00124
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TCCACTTTAAAGAGACACGCT AACCGACCCCTTTCAAAAGA 42.9 45.0 57.3 57.3 2443-2479 bp 1.0 60.7
ndhF_p2 TCCACTTTAAAGAGACACGCT ACCGACCCCTTTCAAAAGAA 42.9 45.0 57.3 57.3 2442-2478 bp 1.0 60.7
ndhF_p3 TCCACTTTAAAGAGACACGCT AACCGACCCCTTTCAAAAGAA 42.9 42.9 57.3 58.0 2443-2479 bp 1.0 58.4
ndhF_p4 TCCACTTTAAAGAGACACGCT ACCGACCCCTTTCAAAAGAAA 42.9 42.9 57.3 58.0 2442-2478 bp 1.0 58.4
ndhF_p5 TTCCACTTTAAAGAGACACGCT ACCGACCCCTTTCAAAAGAA 40.9 45.0 57.9 57.3 2443-2479 bp 1.0 58.1