Hotspot

rpl20-rps12

LSC · 71605-72392 · 788 bp

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Pi 0.00127
MarkerSeek score 59.90
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
788
variable_sites
2
pi
0.00127
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA CTCAACGACGAGGAACATGT 50.0 50.0 57.9 57.9 907-910 bp 1.0 78.9
rpl20-rps12_p2 TGAATGAGCCCCTCGAAAAG CTCAACGACGAGGAACATGT 50.0 50.0 57.9 57.9 905-908 bp 1.0 78.9
rpl20-rps12_p3 GAATGAGCCCCTCGAAAAGT CTCAACGACGAGGAACATGT 50.0 50.0 57.9 57.9 904-907 bp 1.0 78.9
rpl20-rps12_p4 GAGCCCCTCGAAAAGTTGAT CTCAACGACGAGGAACATGT 50.0 50.0 57.9 57.9 900-903 bp 1.0 78.9
rpl20-rps12_p5 AGCCCCTCGAAAAGTTGATG CTCAACGACGAGGAACATGT 50.0 50.0 58.2 57.9 899-902 bp 1.0 78.4