Hotspot

psbE-petL

LSC · 67096-68390 · 1295 bp

Back to Dryas

Pi 0.000927
MarkerSeek score 60.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1295
variable_sites
3
pi
0.000927
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCG 50.0 55.0 57.8 58.2 1405-1411 bp 1.0 77.9
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC GTCGTATCTTGCTCAGACCG 55.0 55.0 58.2 58.2 1400-1406 bp 1.0 77.6
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC TCGTATCTTGCTCAGACCGA 50.0 50.0 57.8 58.3 1404-1410 bp 1.0 77.2
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC TCGTATCTTGCTCAGACCGA 55.0 50.0 58.2 58.3 1399-1405 bp 1.0 76.9
psbE-petL_p5 TCTCCTGTGCTTCCAGACAT GTCGTATCTTGCTCAGACCG 50.0 55.0 58.4 58.2 1398-1404 bp 1.0 76.3