Hotspot

trnF-GAA-ndhJ

LSC · 50879-51686 · 808 bp

Back to Dryas

Pi 0.000998
MarkerSeek score 56.90
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
808
variable_sites
2
pi
0.000998
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA ATCCACGCCTGAAACGTATC 55.0 50.0 58.0 58.1 955-983 bp 1.0 82.3
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATCCACGCCTGAAACGTATC 50.0 50.0 58.1 58.1 919-947 bp 1.0 82.0
trnF-GAA-ndhJ_p3 TCAAATCTGGTTCCTGGCAC ATCCACGCCTGAAACGTATC 50.0 50.0 58.1 58.1 904-932 bp 1.0 81.7
trnF-GAA-ndhJ_p4 GTCGTGAATGGTCGGGATAG ATCCACGCCTGAAACGTATC 55.0 50.0 57.9 58.1 968-996 bp 1.0 81.2
trnF-GAA-ndhJ_p5 CTGGTAGAGCAGAGGACTGA ATCCACGCCTGAAACGTATC 55.0 50.0 57.9 58.1 943-971 bp 1.0 81.2