Hotspot

rpoB-trnC-GCA

LSC · 27361-28591 · 1231 bp

Back to Dryas

Pi 0.000651
MarkerSeek score 60.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1231
variable_sites
2
pi
0.000651
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCATTTTCACCCCCAAGCAT CCGGATTTGAACTGGGGAAA 45.0 50.0 57.7 58.1 1313-1337 bp 1.0 77.9
rpoB-trnC-GCA_p2 CTCATTTTCACCCCCAAGCA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.4 58.1 1314-1338 bp 1.0 77.5
rpoB-trnC-GCA_p3 ATTGCCTCATTTTCACCCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 58.4 58.1 1319-1343 bp 1.0 77.1
rpoB-trnC-GCA_p4 TCATTTTCACCCCCAAGCAT TCTTTTGGTGATCAGGCGAC 45.0 50.0 57.7 58.2 1335-1359 bp 1.0 77.0
rpoB-trnC-GCA_p5 CTCATTTTCACCCCCAAGCA TCTTTTGGTGATCAGGCGAC 50.0 50.0 58.4 58.2 1336-1360 bp 1.0 76.6