Hotspot

trnR-UCU-atpA

LSC · 9927-10419 · 493 bp

Back to Dryas

Pi 0.00385
MarkerSeek score 64.40
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
493
variable_sites
4
pi
0.00385
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 TGGAATGAAAAGCGTCCATTG GAGGAAGCAGAAGCCCTTTT 42.9 50.0 57.4 58.1 595-661 bp 1.0 71.2
trnR-UCU-atpA_p2 GGAATGAAAAGCGTCCATTGT GAGGAAGCAGAAGCCCTTTT 42.9 50.0 57.4 58.1 594-660 bp 1.0 71.2
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC GAGGAAGCAGAAGCCCTTTT 47.6 50.0 57.3 58.1 534-600 bp 1.0 70.3
trnR-UCU-atpA_p4 TGGAATGAAAAGCGTCCATTG AGGAAGCAGAAGCCCTTTTG 42.9 50.0 57.4 58.4 594-660 bp 1.0 68.9
trnR-UCU-atpA_p5 GGAATGAAAAGCGTCCATTGT AGGAAGCAGAAGCCCTTTTG 42.9 50.0 57.4 58.4 593-659 bp 1.0 68.9