Hotspot

psbB

LSC · 74324-75850 · 1527 bp

Back to Drummondita

Pi 0.00349

MarkerSeek score 48.60

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 8
pi: 0.00349
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	AAAAAGAAGGGAGGGCACAG	ACCCAAAATAGTGGAGCAGT	50.0	45.0	58.0	56.7	1640 bp	1.0	70.2
psbB_p2	AAAAAGAAGGGAGGGCACAG	AACCCAAAATAGTGGAGCAGT	50.0	42.9	58.0	57.5	1641 bp	1.0	68.2
psbB_p3	AAAAAGAAGGGAGGGCACAG	ACCCAAAATAGTGGAGCAGTAC	50.0	45.5	58.0	58.0	1640 bp	1.0	64.0
psbB_p4	AAAAGAAGGGAGGGCACAGA	ACCCAAAATAGTGGAGCAGT	50.0	45.0	58.9	56.7	1639 bp	1.0	63.4
psbB_p5	AAAAAGAAGGGAGGGCACAG	AAACCCAAAATAGTGGAGCAGT	50.0	40.9	58.0	58.2	1642 bp	1.0	63.1