Hotspot

psbA

LSC · 457-1518 · 1062 bp

Back to Drummondita

Pi 0.00126

MarkerSeek score 34.10

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCGTCTACACAAAAACCA	GATGATTAAATAAACCAAGATTTTACC	40.0	25.9	54.9	54.0	1114 bp	1.0	40.1
psbA_p2	AACTCGTCTACACAAAAACCA	GATGATTAAATAAACCAAGATTTTACC	38.1	25.9	55.7	54.0	1115 bp	1.0	40.1
psbA_p3	ACTCGTCTACACAAAAACCAA	GATGATTAAATAAACCAAGATTTTACC	38.1	25.9	55.7	54.0	1114 bp	1.0	40.1
psbA_p4	AACTCGTCTACACAAAAACCAA	GATGATTAAATAAACCAAGATTTTACC	36.4	25.9	56.4	54.0	1115 bp	1.0	40.1
psbA_p5	ACTCGTCTACACAAAAACCAAA	GATGATTAAATAAACCAAGATTTTACC	36.4	25.9	56.4	54.0	1114 bp	1.0	40.1