Hotspot

trnR-ACG-trnN-GUU

IRb · 110335-110965 · 631 bp

Back to Drummondita

Pi 0.00106

MarkerSeek score 62.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 631
variable_sites: 1
pi: 0.00106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	TAGGGTACTTCCGGGAACAG	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.1	58.1	848-864 bp	1.0	79.9
trnR-ACG-trnN-GUU_p2	TAGGGTACTTCCGGGAACAG	GCTGTTAACTGACTGGTCGT	55.0	50.0	58.1	57.8	771-787 bp	1.0	79.6
trnR-ACG-trnN-GUU_p3	TAGGGTACTTCCGGGAACAG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.1	58.2	758-774 bp	1.0	79.6
trnR-ACG-trnN-GUU_p4	TAGGGTACTTCCGGGAACAG	TAGAATCTCCCGCTGTTCCT	55.0	50.0	58.1	57.8	816-832 bp	1.0	79.4
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	797-813 bp	1.0	79.4