Hotspot

psbT-psbN

LSC · 76156-76226 · 71 bp

Back to Drummondita

Pi 0.0282

MarkerSeek score 67.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 3
pi: 0.0282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	50.0	55.0	57.6	58.4	131-133 bp	1.0	76.1
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	GATCCATTCGAGGAACACGG	50.0	55.0	57.5	58.4	129-131 bp	1.0	75.3
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	AGATCCATTCGAGGAACACG	50.0	50.0	57.6	57.4	132-134 bp	1.0	74.8
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	GGCAACCCTCTCAACAACTA	50.0	50.0	57.6	57.4	154-156 bp	1.0	74.7
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	50.0	50.0	57.5	57.4	152-154 bp	1.0	73.9