Hotspot

petA-psbJ

LSC · 64427-65510 · 1084 bp

Back to Drummondita

Pi 0.008

MarkerSeek score 64.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1084
variable_sites: 13
pi: 0.008
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.0	58.1	1224-1228 bp	1.0	80.6
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TCCGGATTAGGTTCGTCCTT	50.0	50.0	58.0	58.1	1225-1229 bp	1.0	80.0
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.2	58.1	1221-1225 bp	1.0	79.5
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1189-1193 bp	1.0	79.2
petA-psbJ_p5	TTGGCATCTGTTGTTTTGGC	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1192-1196 bp	1.0	79.1