Hotspot

ycf4-cemA

LSC · 62025-62525 · 501 bp

Back to Drummondita

Pi 0.00937

MarkerSeek score 63.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 7
pi: 0.00937
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	ATGAGAGAGGGATCCACCAG	45.0	55.0	58.1	57.9	632-634 bp	1.0	79.7
ycf4-cemA_p2	TGCGTGTACCAATTGAAGGT	ATGAGAGAGGGATCCACCAG	45.0	55.0	57.7	57.9	619-621 bp	1.0	78.4
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	TAAGGGGAATGGATGCGTTC	45.0	50.0	58.1	57.7	575-577 bp	1.0	77.9
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	CTAAGGGGAATGGATGCGTT	45.0	50.0	58.1	57.7	576-578 bp	1.0	77.8
ycf4-cemA_p5	GGCCATTCCCTTGAATCGTA	ATGAGAGAGGGATCCACCAG	50.0	55.0	57.7	57.9	699-701 bp	1.0	77.6