Hotspot

psaI-ycf4

LSC · 61020-61469 · 450 bp

Back to Drummondita

Pi 0.0105

MarkerSeek score 51.40

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 7
pi: 0.0105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATGGCAATGGCTTCTTT	ATATTCTGATCGCCAACTCA	45.0	40.0	57.9	54.2	519-525 bp	1.0	51.1
psaI-ycf4_p2	GCAATGGCAATGGCTTCTTT	TATTCTGATCGCCAACTCAT	45.0	40.0	57.9	54.2	518-524 bp	1.0	51.1
psaI-ycf4_p3	AGTATTTCCGGCAATGGCAA	ATATTCTGATCGCCAACTCA	45.0	40.0	58.2	54.2	529-535 bp	1.0	50.7
psaI-ycf4_p4	AGTATTTCCGGCAATGGCAA	TATTCTGATCGCCAACTCAT	45.0	40.0	58.2	54.2	528-534 bp	1.0	50.7
psaI-ycf4_p5	GGCCTAGTATTTCCGGCAAT	TATTCTGATCGCCAACTCAT	50.0	40.0	57.7	54.2	533-539 bp	1.0	49.7