Hotspot

psbZ-trnG-GCC

LSC · 37595-38114 · 520 bp

Back to Drummondita

Pi 0.0237

MarkerSeek score 72.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 520
variable_sites: 18
pi: 0.0237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.4	58.1	679-682 bp	1.0	78.9
psbZ-trnG-GCC_p2	CGGATGGTTGGTCGAGTAAC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.4	58.1	680-683 bp	1.0	78.9
psbZ-trnG-GCC_p3	CGGATGGTTGGTCGAGTAAC	TTCCTTGACTCTGGCGGATA	55.0	50.0	58.4	58.1	704-707 bp	1.0	78.1
psbZ-trnG-GCC_p4	TTTGCTTCTCCGGATGGTTG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.5	58.1	689-692 bp	1.0	78.0
psbZ-trnG-GCC_p5	TTTGCTTCTCCGGATGGTTG	GAATCGAACCCGCATCTTCT	50.0	50.0	58.5	58.1	690-693 bp	1.0	78.0