Hotspot

psbC-trnS-UGA

LSC · 36711-36963 · 253 bp

Back to Drummondita

Pi 0.0397

MarkerSeek score 79.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 253
variable_sites: 15
pi: 0.0397
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCAGCAGGGTTTGAAAAAGG	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.1	57.9	390-394 bp	1.0	82.0
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	341-345 bp	1.0	81.9
psbC-trnS-UGA_p3	CCTGTTCTTTCGATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.3	57.9	351-355 bp	1.0	81.4
psbC-trnS-UGA_p4	CCTGTTCTTTCGATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.3	58.2	302-306 bp	1.0	81.4
psbC-trnS-UGA_p5	GCAGCAGGGTTTGAAAAAGG	GATAGCCCCGGTCTTGAAAA	50.0	50.0	58.1	57.6	391-395 bp	1.0	79.7