Hotspot

trnT-GGU-psbD

LSC · 33037-34279 · 1243 bp

Back to Drummondita

Pi 0.00644
MarkerSeek score 62.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1243
variable_sites
12
pi
0.00644
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AATTGAACCAAATGGCCCCT GAATCGGTCCCTTCGTAACC 45.0 55.0 58.0 58.1 1406-1435 bp 1.0 80.2
trnT-GGU-psbD_p2 ATTGAACCAAATGGCCCCTT GAATCGGTCCCTTCGTAACC 45.0 55.0 58.0 58.1 1405-1434 bp 1.0 80.2
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG GAATCGGTCCCTTCGTAACC 55.0 55.0 58.1 58.1 1378-1407 bp 1.0 80.0
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GAATCGGTCCCTTCGTAACC 50.0 55.0 58.1 58.1 1381-1410 bp 1.0 79.9
trnT-GGU-psbD_p5 ATTGAACCAAATGGCCCCTT GGACCAGCCTACAAAAACGA 45.0 50.0 58.0 58.1 1423-1452 bp 1.0 79.8