Hotspot

trnD-GUC-trnY-GUA

LSC · 31442-31907 · 466 bp

Back to Drummondita

Pi 0.01
MarkerSeek score 56.40
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
466
variable_sites
7
pi
0.01
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 536-538 bp 1.0 75.3
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 535-537 bp 1.0 75.3
trnD-GUC-trnY-GUA_p3 TTTGATTTGGCTCCATCGGG TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 596-598 bp 1.0 74.8
trnD-GUC-trnY-GUA_p4 TTTGATTTGGCTCCATCGGG GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 595-597 bp 1.0 74.8
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 557-559 bp 1.0 74.4