Hotspot

psbM-trnD-GUC

LSC · 30782-31367 · 586 bp

Back to Drummondita

Pi 0.00456

MarkerSeek score 61.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 586
variable_sites: 4
pi: 0.00456
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCAGTAGGAACTAGAACGA	TTTTACTTGCCCGGGATTGT	47.6	45.0	57.9	57.7	733-749 bp	1.0	69.5
psbM-trnD-GUC_p2	AGGCAGTAGGAACTAGAACGA	TACTTGCCCGGGATTGTAGT	47.6	50.0	57.9	58.4	730-746 bp	1.0	68.5
psbM-trnD-GUC_p3	AGCAAAAAGGCAGTAGGAACT	TTTTACTTGCCCGGGATTGT	42.9	45.0	57.8	57.7	740-756 bp	1.0	68.3
psbM-trnD-GUC_p4	AGGCAGTAGGAACTAGAACGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.9	58.5	711-727 bp	1.0	68.1
psbM-trnD-GUC_p5	AGCAAAAAGGCAGTAGGAACT	TACTTGCCCGGGATTGTAGT	42.9	50.0	57.8	58.4	737-753 bp	1.0	67.3