Hotspot

psbA-trnK-UUU

LSC · 1519-1785 · 267 bp

Back to Drummondita

Pi 0.0262

MarkerSeek score 67.40

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 10
pi: 0.0262
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	ATGTAAAGGCGGTTTTCGGT	TGCAATGAAAAATGCGTGCA	45.0	40.0	58.1	58.1	462-464 bp	1.0	80.0
psbA-trnK-UUU_p2	ATGTAAAGGCGGTTTTCGGT	GCAATGAAAAATGCGTGCAC	45.0	45.0	58.1	57.8	461-463 bp	1.0	79.4
psbA-trnK-UUU_p3	ATGTAAAGGCGGTTTTCGGT	GTGCAATGAAAAATGCGTGC	45.0	45.0	58.1	57.8	463-465 bp	1.0	79.4
psbA-trnK-UUU_p4	ACCATCCAATGTAAAGGCGG	TGCAATGAAAAATGCGTGCA	50.0	40.0	58.2	58.1	470-472 bp	1.0	78.9
psbA-trnK-UUU_p5	CCATCCAATGTAAAGGCGGT	TGCAATGAAAAATGCGTGCA	50.0	40.0	58.2	58.1	469-471 bp	1.0	78.9