Hotspot

ycf1

SSC · 126196-131808 · 5613 bp

Back to Driessenia

Pi 0.0103

MarkerSeek score 47.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5613
variable_sites: 84
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGTGTAGTGATTCTCGGAC	CCTTTTGGTTCTCCCCGAAA	45.5	50.0	57.8	58.0	536-680 bp	1.0	68.0
ycf1_p2	GTTGTGTAGTGATTCTCGGACT	CCTTTTGGTTCTCCCCGAAA	45.5	50.0	57.8	58.0	535-679 bp	1.0	68.0
ycf1_p3	TGAATGGGAATGGTAAGGGATG	CCTTTTGGTTCTCCCCGAAA	45.5	50.0	57.8	58.0	200-344 bp	1.0	68.0
ycf1_p4	TGAATGGGAATGGTAAGGGA	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	55.8	58.0	200-344 bp	1.0	67.7
ycf1_p5	GTTGTGTAGTGATTCTCGGACT	CCTTCCTTTTGGTTCTCCCC	45.5	55.0	57.8	58.1	539-683 bp	1.0	67.5