Hotspot

ccsA-ndhD

SSC · 117647-117967 · 321 bp

Back to Driessenia

Pi 0.0355

MarkerSeek score 65.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 321
variable_sites: 16
pi: 0.0355
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGCAGCTCTTTTATGCGGA	TCAATGGCTTCCCTTGCATT	45.0	45.0	58.0	58.1	1191-1202 bp	1.0	82.4
ccsA-ndhD_p2	TGCAGCTCTTTTATGCGGAT	TCAATGGCTTCCCTTGCATT	45.0	45.0	58.0	58.1	1190-1201 bp	1.0	82.4
ccsA-ndhD_p3	TGCAGCTCTTTTATGCGGAT	TTTTGATTCTGGGCCGAGAG	45.0	50.0	58.0	57.9	972-983 bp	1.0	82.4
ccsA-ndhD_p4	TTAGTACCCGCGCTTCAATC	TCAATGGCTTCCCTTGCATT	50.0	45.0	58.1	58.1	1252-1263 bp	1.0	82.0
ccsA-ndhD_p5	TGCAGCTCTTTTATGCGGAT	CTCGACAACATGGGTGGAAT	45.0	50.0	58.0	57.9	1253-1264 bp	1.0	81.9