Hotspot

ndhF-rpl32

SSC · 115187-116088 · 902 bp

Back to Driessenia

Pi 0.0312

MarkerSeek score 69.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 902
variable_sites: 41
pi: 0.0312
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1408-1531 bp	1.0	83.5
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1409-1532 bp	1.0	83.5
ndhF-rpl32_p3	AGCATAATCGAAGCAAGCGA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	1646-1769 bp	1.0	83.0
ndhF-rpl32_p4	TGGCAACTGCACCTGTAAAT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	2074-2197 bp	1.0	82.9
ndhF-rpl32_p5	TGGCAACTGCACCTGTAAAT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	2075-2198 bp	1.0	82.9