Hotspot

rpl20-rps12

LSC · 70594-71439 · 846 bp

Back to Driessenia

Pi 0.015

MarkerSeek score 57.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 19
pi: 0.015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CACGAAAGCTTGACGCAAAT	CAGCGACGAGGAACATGTAC	45.0	55.0	58.0	58.7	952-961 bp	1.0	76.3
rpl20-rps12_p2	CACGAAAGCTTGACGCAAAT	AGCGACGAGGAACATGTACT	45.0	50.0	58.0	58.8	951-960 bp	1.0	75.5
rpl20-rps12_p3	CACGAAAGCTTGACGCAAAT	TCAGCGACGAGGAACATGTA	45.0	50.0	58.0	58.8	953-962 bp	1.0	75.5
rpl20-rps12_p4	CACGAAAGCTTGACGCAAAT	GCGACGAGGAACATGTACTA	45.0	50.0	58.0	57.2	950-959 bp	1.0	75.4
rpl20-rps12_p5	TGTTCGACGTCTACGAGCTA	CAGCGACGAGGAACATGTAC	50.0	55.0	58.3	58.7	921-930 bp	1.0	74.4