Hotspot

petG-trnW-CCA

LSC · 67569-67703 · 135 bp

Back to Driessenia

Pi 0.0207

MarkerSeek score 65.80

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 4
pi: 0.0207
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACGTGGTGATCAGTTAGACC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.0	58.0	213-214 bp	1.0	75.2
petG-trnW-CCA_p2	ACGTGGTGATCAGTTAGACC	CAGAACGTGGGTCTCCAAAA	50.0	50.0	57.0	58.0	214-215 bp	1.0	75.2
petG-trnW-CCA_p3	ACGTGGTGATCAGTTAGACC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.0	58.0	235-236 bp	1.0	75.0
petG-trnW-CCA_p4	ACGTGGTGATCAGTTAGACC	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.0	57.9	199-200 bp	1.0	75.0
petG-trnW-CCA_p5	ACGTGGTGATCAGTTAGACC	GCTCTTAGTTCAGTTCGGCA	50.0	50.0	57.0	57.9	232-233 bp	1.0	74.5