Hotspot

psbE-petL

LSC · 66017-67171 · 1155 bp

Back to Driessenia

Pi 0.0205

MarkerSeek score 58.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 35
pi: 0.0205
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TTTATCTTGCTTCCTCCGCC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2443-2457 bp	1.0	82.1
psbE-petL_p2	CTTCCTCCGCCATTTCATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2434-2448 bp	1.0	82.1
psbE-petL_p3	TCCTCCGCCATTTCATCAAG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2432-2446 bp	1.0	82.1
psbE-petL_p4	CGGTAGGTACAGCTAGTCCA	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	2090-2112 bp	1.0	82.0
psbE-petL_p5	TCGTTCGGGTTTGATTGTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2182-2204 bp	1.0	81.9